Composite

Part:BBa_K2443031:Design

Designed by: Lethbridge iGEM 2017   Group: iGEM17_Lethbridge   (2017-10-18)


RF3 optimized for expression in E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 919
    Illegal AgeI site found at 980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1143


Design Notes

Codon optimized for use in Escherichia coli. All regulatory elements are also optimal for use within Escherichia coli



Source

see basic parts

References